关键词: 草乌头, 转录组, 遮荫, qRT-PCR

Abstract: The effects of shading treatment on differentially expressed genes in leaves of Aconitum kusnezoffii were analyzed to reveal the molecular mechanism of its responses to shading. Through the transcriptome sequencing of the leaves of A. kusnezoffii after shading treatment,it was found that the data quality was high (Q30 ≥ 94.37 %),and 59 082 Unigenes (N50 = 1 826 bp) were obtained by splicing and assembly. Functional annotation showed that 27 668 Unigenes (46.83 %) were annotated to multiple important databases. Analysis of differentially expressed genes found 483 differentially expressed genes,of which 421 were annotated. It was found that light conditions significantly affected the biological processes such as photosynthesis,hormone regulation and energy metabolism of A. kusnezoffii. Key gene analysis showed that LHCB17,ATPβ and PetA were down-regulated under shading,LHCB1 and GA2ox were up-regulated. The regulation of these genes revealed the effect of light on the growth and metabolic adaptability of A. kusnezoffii. Real-time fluorescence quantitative analysis verified the reliability of transcriptome data.The results of this study not only enhance the understanding of the adaptation of A. kusnezoffii to low light environment,but also provide clues for the study of its genetic regulatory network and functional genes,which is of great significance for the rational cultivation and utilization of A. kusnezoffii.

Key words: Aconitum kusenezoffii L., Transcriptome, Shade, qRT-PCR