西藏野生垂穗披碱草CnCOR410基因克隆及表达分析

doi:10.11733/j.issn.1007-0435.2025.07.005

草地学报 ?? 2025, Vol. 33 ?? Issue (7): 2090-2098.DOI: 10.11733/j.issn.1007-0435.2025.07.005

西藏野生垂穗披碱草CnCOR410基因克隆及表达分析

柴艺¹, 饶鑫媛¹, 袁璐瑶¹, 王鹤潼¹, 张博宁¹, 苗彦军², 曹仲华², 杨培志¹, 呼天明¹, 付娟娟¹

1. 西北农林科技大学草业与草原学院, 陕西杨凌 712100;
2. 西藏农牧学院动物科学学院, 西藏林芝 860000

收稿日期:2025-08-04 修回日期:2025-08-04 发布日期:2025-08-04
通讯作者: 呼天明,E-mail:hutianming@126.com;付娟娟,E-mail:fujuanjuan1234@126.com
作者简介:柴艺（1999-），女，汉族，河北沧州人，硕士研究生，主要从事牧草逆境生物学研究，E-mail：535247614@qq.com
基金资助:
国家自然科学基金-区域创新发展联合基金（U21A20240）；国家自然科学基金地区基金（32260342）资助

Cloning and Expression Analysis of the CnCOR410 Gene in Campeiostachys nutans Griseb.

CHAI Yi¹, RAO Xin-yuan¹, YUAN Lu-yao¹, WANG He-tong¹, ZHANG Bo-ning¹, MIAO Yan-jun², CAO Zhong-hua², YANG Pei-zhi¹, HU Tian-ming¹, FU Juan-juan¹

1. Department of Grassland Science, College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. College of Animal Science, Agricultural and Animal Husbandry College of Tibet University, Nyingchi, Xizang 860000, China

Received:2025-08-04 Revised:2025-08-04 Published:2025-08-04

摘要/Abstract

摘要： 为了研究脱水素调控植物非生物胁迫应答的分子机制，本研究从西藏野生垂穗披碱草（Campeiostachys nutans Griseb.）中克隆了CnCOR410基因，并进行了生物信息学、基因表达模式和亚细胞定位分析。结果表明：CnCOR410序列全长765 bp，编码254个氨基酸，分子量为7504.48 Da，等电点为4.87；CnCOR410蛋白属于亲水蛋白、非跨膜蛋白；二级结构以无规则卷曲为主，占比61.42%；CnCOR410包含脱水素保守结构域属于LEA家族；CnCOR410与小麦（Triticum aestivum L.）的氨基酸序列相似性最高为93.44%，且该蛋白定位在细胞质上；CnCOR410在垂穗披碱草根、茎、叶组织中均有表达，在叶片中表达量最高；且CnCOR410表达受低温、干旱、盐胁迫和ABA调控（P<0.05）。本研究结果为深入探明CnCOR410调控垂穗披碱草非生物胁迫适应机制奠定了基础。

关键词: 垂穗披碱草, 脱水素, CnCOR410, 基因克隆, 表达响应, 西藏

Abstract: To investigate the molecular mechanism of dehydrin regulating plant abiotic stress response， the CnCOR410 gene was cloned from Xizang wild Campeiostachys nutans Griseb. Amino acid sequence analysis， subcellular localization， and expression profile of the CnCOR410 gene in response to cold， drought， and salt stress were conducted. The results showed that the coding sequences （CDS） oftheCnCOR410 gene was 765 bp in length， encoding 254 amino acids， with a molecular weight of 7504.48 Da and an isoelectric point of 4.87. The CnCOR410 protein was a hydrophilic and non-transmembrane protein， and its secondary structure was mainly random coil， accounting for 61.42%. The CnCOR410 contained the conserved domain of dehydrin and belonged to the LEA family. The amino acid sequence similarity between CnCOR410 and wheat （Triticum aestivum L） was the highest at 93.44%， and the protein was located in the cytoplasm. The RT-qPCR results showed that CnCOR410 was expressed in the root， stem， and leaf tissues of C. nutans， with the highest expression in the leaves. Moreover， the expression of CnCOR410 was regulated by cold， drought， salt stress， and ABA （P<0.05）. The results of this study laid a foundation for further clarifying the mechanism of CnCOR410 regulating the abiotic stress adaptation of C. nutans.

Key words: Campeiostachys nutans Griseb., Dehydrin, CnCOR410, Gene cloning, Expression analysis, Xizang

中图分类号:

S543.9

柴艺, 饶鑫媛, 袁璐瑶, 王鹤潼, 张博宁, 苗彦军, 曹仲华, 杨培志, 呼天明, 付娟娟. 西藏野生垂穗披碱草CnCOR410基因克隆及表达分析[J]. 草地学报, 2025, 33(7): 2090-2098.

CHAI Yi, RAO Xin-yuan, YUAN Lu-yao, WANG He-tong, ZHANG Bo-ning, MIAO Yan-jun, CAO Zhong-hua, YANG Pei-zhi, HU Tian-ming, FU Juan-juan. Cloning and Expression Analysis of the CnCOR410 Gene in Campeiostachys nutans Griseb.[J]. Acta Agrestia Sinica, 2025, 33(7): 2090-2098.

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西藏野生垂穗披碱草CnCOR410基因克隆及表达分析

Cloning and Expression Analysis of the CnCOR410 Gene in Campeiostachys nutans Griseb.

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